Induksi Planlet Anggrek Cattlyea Sp Secara In-Vitro Pada Media Murashige-Skoog Dan Bahan Organik

Ida Royani

Abstract


The background of this study is the high price of synthetic growth regulators which is one of the obstacles in plant propagation in vitro. This study aims to obtain the right formula by using carrot extract and coconut water (organic material) in the induction of Cattleya sp orchid plantlets in vitro. This research was carried out at the West Java West Nusa Tenggara BBI-PPH Network Culture Laboratory. The study was conducted using a factorial randomized block design (RBD) with two factors, each factor consisting of 3 levels. Factor 1, MS (M) Media Concentration with 3 levels: M1: MS Full; M2: ½ ms; M3: ¼ MS. Factor 2, Organic Composition (O) consists of: O1: Carrot Extract 40 ml / L + Coconut water 150 ml / L; O2: Carrot extract 50 ml / L + Coconut water 200 ml / L; O3: Carrot extract 60 ml / L + 250 ml coconut water / L. The results of research on the parameters of the most number of leaves found in the treatment of MS full + 40 ml / L carrot extract + 150 ml / L coconut water with an average of 5.44 leaves. The highest number of shoots was found in the treatment of 1/2 MS + 40 ml / L carrot extract + 150 ml / L coconut water with an average of 3.49. The highest number of roots was found in the treatment of ½ MS + 40 ml / L carrot extract + 150 ml / L coconut water with an average of 12.06

Keywords


Planlet Induction; MS; Organic Material; In-Vitro

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References


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